目的:测定民族药结石草不同药用部位中车前醚苷的含量,建立民族药结石草车前醚苷的HPLC(High Performance Liquid Chromatography)检测方法.方法:采用PntulipsTM BP C18(4.6mm×250mm,5μmn)色谱柱,以甲醇∶水-(60∶40)为流动相,流速为1.0 mL·min-1,检测波长241 nm,柱温30℃.结果:车前醚苷的线性范围为:0.352～4.224μg,回归方程为:Y =0.0752X +0.0007(r=0.9999),平均加样回收率100.78％,RSD值为1.83％(N=6).结论:该法专属性强、快速、简便、准确,可为结石草中车前醚苷质量控制方法提供依据.
Determination of the Contents of Plantarenaloside in Different Parts of Incarvillea arguta by HPLC
Objective:To determine the content of plantarenaloside in different parts of ethnomedicine Incarvillea arguta and establish HPLC (High Performance Liquid Chromatography) detection methods for this chemical component.Method:Pntulips TM BP C18(4.6 mm ×250 mm,5 μm)was used as a mobile phase consisting of methanel-water (60∶40).The flow rate was 1.0 mL · min-1.The detection wavelength was 241 nm.The column temperature was 30℃.Result:The linear range of Plantarenaloside was 0.352 ～4.224μg and the linear equation was y =0.0752x +0.0007(r =0.9999).The average sample recovery rate was 100.78％,and RSD (％) was 1.83.％ (n =6).Conclusion:The method is quick,simple and accurate.It can provide a reference for quality control of plantarenaloside in Incarvillea arguta.
Incarvillea argutaPlantarenalosideHPLC methodQuality control
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维普
