Effect of Xinshuaining Granule on Transforming Growth Factor Beta 1 of Rats with Chronic Heart Failure
Objective:To discuss the influence of expression of Xinshuaining Granule on transforming growth factor beta 1 (TGF-β1) of rats with chronic heart failure.Methods:SD rats were divided into a normal group,a model group,a group with Captopril,a group with high-dose Xinshuaining granule,a group with medium-dose Xinshuaining granule and a group with low-dose Xinshuaining granule.Except the normal group,SD rats in other groups were induced to get chronic heart failure by Intraperitoneal injection with adriamycin,while the rats in the normal group were injected with equivalent normal saline once a week for 6 weeks.After successfully establishing the model,each group was given corresponding drug intervention respectively for 3 weeks.Then,echocardiography was used to test the cardiac function.The levels of proBNP and TGF-β1 in rats serum were tested.Using the qRT-PCR and western blot detected the gene and protein expression level of TGF-β1.Methods:Compared with the normal group,the left ventricular end diastolic dimension (LVEDD) and left ventricular end systolic dimension(LVESD) increased significantly (P ＜0.01).The left ventricular ejection fraction (LVEF) reduced (P ＜ 0.01),the serum TGF-β1 and proBNP increased significantly (P ＜ 0.01) and the gene and protein expression level of TGF-β1 increased significantly (P ＜ 0.01).Compared with the model group,the group with medium-dose Xinshuaining granule and the group with high-dose Xinshuaining granule could reduce LVEDD and LVESD (P ＜ 0.05),improved the LVEF of rats with chronic heart failure (P ＜0.01),reduced the level of serum TGF-β1 and proBNP(P ＜0.01) and reduced the gene and protein expression level of TGF-β1 (P ＜ 0.05).Conclusion:Xinshuaining granule can improve heart function,interpose cardiac remodeling and resist myocardial fibrosis.Its mechanism may be concerned with the inhibiting the TGF -β1 level.
NETL
NSTL
万方数据
维普
