Analysis the Effect of Sophora flavescens Alt on the Number of Escherichia coli in the Gastrointestinal Tract of Rats by Method of Real-time fluorescent Quantitative PCR
Objective:To analyze the effect of Sophora flavescens Alt.on the number of Escherichia coli.in the stomach,duodenum,jejunum,ileum,colon and excrement of SD rats by the method of real-time fluorescent quantitative PCR and study the regulating effect of Sophora flavescens Alt.on escherichia coli bacteria in gastrointestinal tract.Methods:Fed 150 SD rats with conventional way,and gave decoction of Sophora flavescens Alt.by gavage with high,medium and low dose.Meanwhile,a blank control group and an antibiotic group were set up with 10 rats in each group.SD rats' stomach,duodenum,jejunum,ileum parts and colon paragraphs (including their contents) and feces were gathered in the first,second and third weeks,and bacterial genome DNA was extracted.Detected the number of Escherichia coli at different time points of administration by using real-time fluorescent quantitative PCR method.Results:(1) Compared with the blank control group:in the dosage group,the number of Escherichia coli in the duodenal parts decreased significantly at the first,second and third weeks,(P ＜0.01);In the different dose group,at different administration time,the number of Escherichia coli.in the stomach,jejunum,ileum parts,and feces reduced to some extent (P ＜ 0.05).(2) (2) Compared with the antibiotics group:two weeks after administration,the number of Escherichia coli.in duodenum,jejunum and ileum in low,medium and high dose groups reduced significantly (P ＜ 0.05),while in the high dose group,the number of Escherichia coli.in colon parts and feces increased obviously after one week of drug delivery (P ＜0.01).Conclusion:Intragastric administration with Sophora flavescens Alt.decoction can reduce the number of Escherichia coli bacteria in rats' duodenum,jejunum,ileum parts.Its impact on the number of Escherichia coli in duodenal parts decreased gradually over time,which is consistent with the effect of antibiotics.
NETL
NSTL
万方数据
维普
