摘要
目的 探讨扶正化瘀胶囊(FZHY)对肝纤维化发展的影响.方法 CCK-8法检测FZHY对LX-2细胞活性的影响.RT-qPCR和Western blot法测定LX-2转染TGF-β或/和给予FZHY后α-SMA水平.选取2022年1月至2022年12月在上海第九人民医院接受抗病毒治疗的慢性乙型肝炎患者80例作为研究对象,分为对照组和治疗组,治疗组每日口服扶正化瘀胶囊1.5 g,一日3次,瞬时弹性成像检查各组治疗前后瞬时肝弹性F值.结果 FZHY明显抑制LX-2生长,并与剂量呈正相关(P＜0.05).过表达TGF-β后α-SMA mRNA明显增加(57.14±4.81),与对照组相比差异显著(P＜0.001).同时予 10％或 20％FZHY 时,α-SMA mRNA 不同程度降低(37.42±3.65 及 21.79±0.496),与 TGF-β组相比,α-SMA mRNA降低差异有统计学意义(P＜0.05,P＜0.001).Western blot显示,FZHY能部分抵消TGF-β引起的α-SMA表达上调.治疗组瞬时肝弹性F值较干预前有所下降,但差异无统计学意义(P＞0.05).结论 FZHY可通过影响LX-2细胞增殖、调节α-SMA表达来实现抗纤维化作用.
Abstract
Objective To investigate the effect of Fuzheng Huayu Capsules(FZHY)on the progression of hepatic fibrosis.Methods The impact of FZHY on the activity of LX-2 cells was evaluated using the CCK-8 assay.The expression levels of α-SMA in TGF-β-transfected LX-2 cells were assessed using RT-qPCR and Western blot analysis after treatment with or without FZHJ.A total of 80 patients with chronic hepatitis B undergoing antiviral treatment at Shanghai Ninth People's Hospital between January 2022 and December 2022 were enrolled in the study and divided into a control group and a treatment group.The treatment group received 1.5 g of Fuzheng Huayu Capsules three times daily,and liver elasticity F-value were measured using transient elastography before and after treatment in both groups.Results The inhibitory effect of FZHY on LX-2 cells growth was significantly dose-dependen(P＜0.05).Overexpression of TGF-β significantly inceasedα-SMA mRNA level(57.14±4.81)compared to the control group(P＜0.001).Treatment with 10％or 20％FZHY resulted in a reduction in α-SMA mRNA levels(37.42±3.65 and 21.79±0.496)compared to the TGF-β group,with significant differences(P＜0.05,P＜0.001).Western blot analysis demonstrated that FZHY partially reversed the upregulated expression of α-SMA induced by TGF-β.While the liver elasticity F value decreased in the treatment group after therapy,but there was no statistically significant difference(P＞0.05).Conclusion FZHY exhibits antifibrotic effects by influencing LX-2 cell proliferation and modulating α-SMA expression.
基金项目
上海市青年科技英才杨帆计划(19YF1426900)
NETL
NSTL
维普
万方数据