The effect of Fuzheng Huayu Capsules(FZHY)on the development of hepatic fibrosis
Objective To investigate the effect of Fuzheng Huayu Capsules(FZHY)on the progression of hepatic fibrosis.Methods The impact of FZHY on the activity of LX-2 cells was evaluated using the CCK-8 assay.The expression levels of α-SMA in TGF-β-transfected LX-2 cells were assessed using RT-qPCR and Western blot analysis after treatment with or without FZHJ.A total of 80 patients with chronic hepatitis B undergoing antiviral treatment at Shanghai Ninth People's Hospital between January 2022 and December 2022 were enrolled in the study and divided into a control group and a treatment group.The treatment group received 1.5 g of Fuzheng Huayu Capsules three times daily,and liver elasticity F-value were measured using transient elastography before and after treatment in both groups.Results The inhibitory effect of FZHY on LX-2 cells growth was significantly dose-dependen(P＜0.05).Overexpression of TGF-β significantly inceasedα-SMA mRNA level(57.14±4.81)compared to the control group(P＜0.001).Treatment with 10％or 20％FZHY resulted in a reduction in α-SMA mRNA levels(37.42±3.65 and 21.79±0.496)compared to the TGF-β group,with significant differences(P＜0.05,P＜0.001).Western blot analysis demonstrated that FZHY partially reversed the upregulated expression of α-SMA induced by TGF-β.While the liver elasticity F value decreased in the treatment group after therapy,but there was no statistically significant difference(P＞0.05).Conclusion FZHY exhibits antifibrotic effects by influencing LX-2 cell proliferation and modulating α-SMA expression.
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万方数据
维普
