Expression of CD14+ monocytes and CD163+ macrophages in patients with chronic hepatitis C and their relationship with the severity of liver fibrosis
Objective To investigate the expression of CD14+ monocytes and CD163+ macrophages in patients with chronic hepatitis C and their relationship with the severity of liver fibrosis.Methods A total of 83 patients with chronic hepatitis C and 64 healthy subjects were selected between April 2018 and November 2022.The clinical features,the expression of CD163 in liver tissue,the concentration of serum CD163,the proportion of CD14+cells and the proportion of CD14+ cells expressing different kinds of cytokines were compared.Results The level of ALT and AST of chronic hepatitis C group were(84.6±9.1)U/L and(69.3±7.2)U/L,which was significantly higher than that in healthy control group[(28.4±1.7)U/L and(22.5±6.9)U/L](P＜0.05).The concentration of serum CD163 and the proportion of CD14+cells,the number of CD163+cells,concentration of serum CD163+ and CD14+ cell proportion of F＜2 patients were 38.2± 4.7,(73.6±2.1)μg/L and(73.7±3.2)％,which was significantly higher than that in healthy control group[12.5± 1.3,(50.1±2.6)?g/L and(61.0±8.4)％](P＜0.05).The number of CD163+cells,concentration of serum CD163+and CD14+cell proportion of F≥2 patients were 74.9±8.0,(95.4±9.8)μg/,and(79.2±5.5)％,which was significantly higher than that in healthy control group and F＜2 patients.CD14+cells expressing IL-2,IL-4,IFN-γ,IL-6,IL-8 and TNF-α of F＜2 patients were(4.2±1.2)％,(3.8±1.1)％,(3.8±1.0)％,(3.7±1.3)％,(4.0±1.4)％and(6.7±3.6)％,which was significantly higher than that in healthy control group[(0.9±0.1)％,(1.1±0.2)％,(1.8±0.4)％,(1.6±0.6)％,(1.7±0.7)％and(4.6±2.1)％]P＜0.05).CD14+cells expressing IL-2,IFN-y,IL-8 and TNF-α of F≥2 patients were(2.2±0.8)％,(2.1±0.5)％,(5.6±2.0)％and(6.9±3.2)％,which was significantly higher than that in healthy control group.CD14+cells expressing IL-8 was(5.6±2.0)％,which was significantly higher than that in F≥2 patients.Conclusion CD14+ monocytes and CD163+ macrophages are involved in the process of liver fibrosis in patients with chronic hepatitis C.
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