Serum lipidomics analysis in non-obese and obese patients with non-alcoholic fatty liver disease
Objective To compare the serum lipid profiles between non obese and obese patients with non-alcoholic fatty liver disease(NAFLD)patients.Methods A total of 60 NAFLD patients were recruited and categorized into two groups based on body mass index(BMI):non-obese(BMI＜25 kg/m2)and obese(BMI 25 kg/m2).Serum samples from all patients were analyzed using ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS)for lipidomics profiling.Differential lipids were identified through multiple statistical methods,including fold change anaysis,partial least squares discriminant analysis(PLS-DA),and orthogonal partial least squares discriminant analysis(OPLS-DA).Permutation tests were conducted to validate the results.Results A total of 90 differential lipids were identified,Including sphingomyelin(SM),Ether linked phosphatidylcholine(EtherPC),hexosylceramides(HexCer),and phosphatidylethanolamine(PC).KEGG pathway enrichment analysis revealed significant alterations in several metabolic pathways in non-obese NAFLD patients,such as the sphingolipid signaling pathway,necroptosis,glycerophospholipid metabolism pathway,glycosylphosphatidylinositol biosynthetic pathway,and tumor choline metabolism pathway.Conclusion Significant differences in serum lipid mass spectrometry profiles were observed between non-obese and obese NAFLD patients,particularly in PC,PE,SM levels.
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