The expression levels and significance of miR-92a-3p in exosomes,serum and liver tissue of patients with hepatitis B-related liver fibrosis
Objective To investigate the expression levels of microRNA miR-92a-3p in exosomes,serum and liver tissue samples of patients with HBV-related hepatic fibrosis and to analyze the difference and significance.Methods Real-time fluorescent quantitative PCR technology was used to detect the expression levels of miR-92a-3p in exosomes,serum,and liver tissue of patients with hepatitis B-related liver fibrosis at different stages.The Mann-Whitney U test or Kruskal-Wallis test was used to compare the differences in miR-92a-3p expression levels during different stages of liver fibrosis.Spearman's correlation coefficient method was employed to analyze the correlation between the level of miR-92a-3p in exosomes and that in serum and liver tissue.The receiver operating characteristic curve(ROC)was applied to evaluate the predictive efficacy of exosomal and serum miR-92a-3p levels for significant liver fibrosis in hepatitis B.Results The expression level of miR-92a-3p in exosomes showed significant differences among different stages of liver fibrosis(P＜0.05).The expression level of miR-92a-3p in exosomes from patients in S2-4 stages was significantly higher than that in S0-1 stages(P＜0.0001).The relative expression levels of serum miR-92a-3p in S0-1,S2,S3 and S4 stages were 0.69±0.20,1.01±1.35,0.74±0.88 and 0.87±1.33,respectively.There was no statistically significant difference in the expression level of miR-92a-3p in serum among different stages of liver fibrosis(P=0.3817).The relative expression levels of liver tissue miR-92a-3p in S0-1,S2,S3 and S4 stages were 1.69±1.31,1.54±1.26,1.05±0.61 and 0.68±0.54,respectively.Similarly,no statistically significant difference was observed in the expression level of miR-92a-3p in liver tissue among different stages of liver fibrosis(P=0.1040).The expression level of miR-92a-3p in exosomes did not show a significant correlation with its expression level in serum(r=0.1787,P=0.1083).Similarly,there was no significant correlation between the expression levels of miR-92a-3p in exosomes and liver tissue(r=0.051,P=0.7311).When the cut-off value was 0.245,the AUROC for serum miR-92a-3p was 0.61(95％CI:0.48～0.75),with a sensitivity and specificity for diagnosing significant liver fibrosis of 63.46％and 60％,respectively.For exosomal miR-92a-3p,at a cut-off value of 0.7459,the AUROC was highest at 0.87(95％CI:0.80～0.94),with a sensitivity and specificity for diagnosing significant liver fibrosis of 84.13％and 72.09％,respectively.Conclusion The expression levels of miR-92a-3p in exosomes,serum,and liver tissue showed no significant correlation.The concentration of miR-92a-3p in exosomes is significantly higher than in serum and liver tissue,indicating that circulating miR-92a-3p is enriched in exosomes.Compared to serum miR-92a-3p,exosomal miR-92a-3p demonstrated higher diagnostic efficacy for significant liver fibrosis in hepatitis B patients.
万方数据
维普
