Clinical significance and determinants of T lymphocyte mitochondrial damage index in autoimmune hepatitis patients
Objective To investigate the clinical relevance of the T-lymphocyte mitochondrial damage index in autoimmune hepatitis patients and to identify factors influencing T-lymphocyte mitochondrial damage.Methods A total of 60 patients with autoimmune hepatitis admitted to our hospital between October 2021 and October 2023 were enrolled in this study.Levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBil),and albumin(ALb)were measured using a Roche cobas 8000 Fully automated biochemical immunoassay analyzer(c701/c702).Antinuclear antibody(ANA)titers and immunoglobulin G(IgG)levels,were assessed via immunofluorescence.Mitochondrial status in peripheral blood T-lymphocytes was evaluated using an Agilent NovoCyte D3000 flow cytometer.Mitochondrial damage in helper T-lymphocytes(Th cells)and suppressor T-lymphocytes(Ts cells)was quatified through a built-in algorithm that compared mitochondrial staining values.Independent risk factors for T-lymphocyte mitochondrial damage in autoimmune hepatitis were analyzed using multifactorial logistic regression.Additionally,receiver operating characteristic(ROC)curve analysis was applied to assess the clinical diagnostic value of T-lymphocyte mitochondrial damage in these patients.Results In the injured group,the positive rates for ALT,ANA titers,IgG,CD4+lymphocyte count,CD8+lymphocyte count,mitochondrial damage rate of Th cells,and mitochondrial damage rate of Ts cells were(142.6±42.5)U/L,93.3％,(19.6±4.8)g/L,(468.8±22.3)× 106/L,(292.6±32.3)× 106/L,86.7％,and 83.3％,respectively.These values were significantly higher than those in the uninjured group,which presented values of(123.5±31.2)U/L,37.5％,(16.2±2.3)g/L,(417.6±32.1)× 106/L,(265.1±29.3)× 106/L,25.0％,and 50.0％,respectively(P＜0.05).Multifactorial logistic regression analysis indicated that elevated ALT,IgG,ANA titer positivity,CD4+lymphocyte count,and CD8+lymphocyte count were independent risk factors for T-lymphocyte mitochondrial damage in autoimmune hepatitis patients(OR=3.438,3.804,3.504,3.114,3.699,P＜0.05).ROC curve analysis demonstrated that mitochondrial damage in both Th cells and Ts cells has high diagnostic value in identifying autoimmune hepatitis.Conclusion ALT,IgG,ANA titer positivity,CD4+lymphocyte count,and CD8+lymphocyte count are independent risk factors for T-lymphocyte mitochondrial damag in autoimmune hepatitis patients.Early detection of these indices is crucial for diagnosing T-lymphocyte mitochondrial damage.Additionally,mitochondrial damage in Th and Ts cells shows high diagnostic value for autoimmune hepatitis and can be used clinically to predic its occurrence.
T lymphocytesMitochondrial damageAutoimmune hepatitisClinical significanceInfluencing factors
万方数据
维普
