HPLC法测定新乐康片相关药材中8种活性成分含量
HPLC Determined the Content of 8 Active Ingredients in Xinlekang Tablets
邓雨荷 1张庆林 2杨明武 3陈伟 3何珺3
作者信息
- 1. 贵州大学药学院,贵州 贵阳 550025;西南特色药用生物资源开发利用教育部工程研究中心,贵州 贵阳 550025
- 2. 山西太原药业有限公司,山西 太原 030021
- 3. 西南特色药用生物资源开发利用教育部工程研究中心,贵州 贵阳 550025
- 折叠
摘要
建立HPLC法同时测定新乐康片中三味原料药材钩藤、酸枣仁和萝芙木中钩藤碱、异钩藤碱、斯皮诺素、酸枣仁皂苷A、酸枣仁皂苷B、育亨宾、阿义吗啉及利血平等 8 种活性成分的含量.采用Titank C18(250 mm×4.6 mm,5 μm)色谱柱,以乙腈-0.4%磷酸水溶液(含 0.05%三乙胺)为流动相,梯度洗脱,检测波长 204 nm,流速为 1.0 mL/min,柱温为 30℃.上述三味药材相关成分之间分离度良好,在λ=204 nm条件下,其在分别的线性范围内与响应值之间的线性关系良好(R2≥0.9990);8 种活性成分的平均加样回收率 98.37%～101.21%,RSD＜2%,仪器精密度、供试品溶液的稳定性与方法重复性均良好.建立的HPLC法可一法对钩藤、酸枣仁和萝芙木三味中药材中 8 种活性成分含量进行测定,为药业在生产新乐康片前期选材上节约了测定时间,提高了生产效率,同时为新乐康片中活性成分HPLC含量测定方法的建立及其质量标准升级提供参考.
Abstract
To establish HPLC method to determine the content of eight active ingredients,namely,alkali,sponine,sinin,sinin A,B,Yohimbin,morpholine and reserpine in Xinlekang tablets.Using Titank C18(250 mm×4.6 mm,5 μm)column,acetonitrile-0.4%aqueous phosphoric acid solution(containing 0.05%triethylamine)as mobile phase,gradient elution,detection wavelength of 204 nm,the flow rate is 1.0 mL/min,and the column temperature is 30℃.The separation between the related components of the three medicinal materials was good,in the linear range(R2≥0.9990);the average sample recovery of the eight active components was 98.37%～101.21%,RSD<2%,the precision of the instrument and the stability of the test solution and the method were good.The HPLC method can measure the content of 8 active ingredients in uncintus,jujube kernel and lufumu,for the pharmaceutical industry saves the determination time,improves the production efficiency,and provides the determination method of HPLC content and its quality standard upgrade.
关键词
钩藤/酸枣仁/萝芙木/钩藤碱/酸枣仁皂苷A/利血平/高效液相色谱Key words
Uncaria rhynchophylla/Ziziphi Spinosae Semen/Rauvolfia/Rhynchophylline/Jujuboside A/Reserpine/high performance liquid chromatography引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据