HPLC Determined the Content of 8 Active Ingredients in Xinlekang Tablets
To establish HPLC method to determine the content of eight active ingredients,namely,alkali,sponine,sinin,sinin A,B,Yohimbin,morpholine and reserpine in Xinlekang tablets.Using Titank C18(250 mm×4.6 mm,5 μm)column,acetonitrile-0.4%aqueous phosphoric acid solution(containing 0.05%triethylamine)as mobile phase,gradient elution,detection wavelength of 204 nm,the flow rate is 1.0 mL/min,and the column temperature is 30℃.The separation between the related components of the three medicinal materials was good,in the linear range(R2≥0.9990);the average sample recovery of the eight active components was 98.37%～101.21%,RSD<2%,the precision of the instrument and the stability of the test solution and the method were good.The HPLC method can measure the content of 8 active ingredients in uncintus,jujube kernel and lufumu,for the pharmaceutical industry saves the determination time,improves the production efficiency,and provides the determination method of HPLC content and its quality standard upgrade.
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