Preparation of Nanometer Gold-cobalt Ion Colorimrtric Probe and Determination of Cysteine
In this paper,ascorbic acid was used to reduce chlorauric acid to prepare gold nanoparticles.In the presence of cobalt ion,cysteine induced gold nanoparticles to agglomerate and the color of the solution changed from red to blue.A new method for determination of cysteine was established.The preparation conditions of gold-cobalt ion colorimetric probe were discussed and the experimental conditions for determination of cysteine were investigated.The results show that:The optimal reaction time and pH of buffer solution were 20 min and 3.0 respectively.The linear regression equation for the determination of cysteine was A725/A520 =0.3965C+0.0133(C:μM),R2=0.9882.The linear range was 1×10-7 mol/L to 1×10-6 mol/L,and the detection limit was 2.2×10-8 mol/L.The recovery rate of urine samples from healthy people was determined by standard addition method,and the recovery rate was 92.8%～95.8%.A simple method for the determination of cysteine was developed.
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