优化液质法测定多种基质中五氯酚残留量
LC-MS for the Determination of Pentachlorophenol Residues in a Variety of Matrices
廖伟杰 1黄兆翔 1高佳敏1
作者信息
- 1. 中科检测技术服务(广州)股份有限公司,广东 广州 510520
- 折叠
摘要
在GB 23200.92-2016《动物源性食品中五氯酚残留量的测定 液相色谱-质谱法》的基础上,改进了前处理方法,使用 1%氨水乙腈提取,QuEChERS 法净化,氮吹后甲醇复溶,并在多种基质中进行了方法学验证.结果表明,方法平均回收率为 72.22%～89.51%;精密度试验中,相对标准偏差(RSD)为 4.23%～10.28%(n=6);在 1～10 μg/L范围内线性关系良好(相关系数r＞0.995);加标量为 1.0 μg/kg的情况下,信噪比>10;定量限小于1.0 μg/kg,满足检测需求.该方法能够节省前处理时间,同时降低成本.
Abstract
On the basis of GB 23200.92-2016"Determination of pentachlorophenol residues in foods of animal origin liquid chromatography-mass spectrometry",the pretreatment conditions were improved,using 1% ammonia water and acetonitrile to extract,QuEChERS method to purify,methanol after nitrogen blowing reconstitute.The results showed that the method could pass the methodological verification in various matrices,and the average recovery rate was 72.22%～89.51%.The precision test was performed separately,and the relative standard deviation(RSD)was 4.23%～10.28%(n=6),the linear relationship in the range(1～10 μg/L)is outstanding(correlation coefficient R>0.995),the quantification limit is 1.0 μg/kg,and the signal-to-noise ratio is greater than 10.The method can save the time spent on pretreatment,and reduce some costs.
关键词
五氯酚/高效液相色谱串联质谱仪/基质效应/方法学验证/QuEChERS法Key words
pentachlorophenol/high performance liquid chromatography tandem mass spectrometer/matrix effect/methodological validation/QuEChERS method引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据