Spherical Au nanoparticles(Au NPs)with ultrasmall size were controllably synthesized in oleylamine/octadecene/PVP system via microreation.The phase structure,composition and morphology of the Au NPs were comprehensively characterized by various analytical techniques,e.g.ultraviolet-visible spectroscopy(UV-Vis),X-ray diffraction(XRD),energy dispersive X-ray spectroscopy(EDX),high-resolution transmission electron microscopy(HR-TEM),and selected area electron diffraction(SAED).Based on these Au NPs,the acute myocardial infarction rapid test kit(AMIRTK)was successfully fabricated through process optimization and its performance was investigated in detail.It was found that as-synthesized Au NPs exhibited a polycrystalline structure and ultrasmall size(4.57±0.3 nm).Obtained AMIRTK with the above Au NPs could achieve the rapid detection and showed a very low limit of detection(0.1 ng/mL)for cardiac myosin binding protein-C(cMyBP-C)as myocardial marker.Meanwhile,the AMIRTK had high specificity,repeatability and stability,indicating a great potential in practice.
关键词
金纳米颗粒/急性心肌梗死/试剂盒/心肌肌球蛋白结合蛋白C/免疫层析
Key words
Au nanoparticle/acute myocardial infarction/test kit/cardiac myosin binding protein-C/immunochromatography
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