Fabrication of Acute Myocardial Infarction Rapid Test Kit Based on Ultrasmall Au Nanoparticals
Spherical Au nanoparticles(Au NPs)with ultrasmall size were controllably synthesized in oleylamine/octadecene/PVP system via microreation.The phase structure,composition and morphology of the Au NPs were comprehensively characterized by various analytical techniques,e.g.ultraviolet-visible spectroscopy(UV-Vis),X-ray diffraction(XRD),energy dispersive X-ray spectroscopy(EDX),high-resolution transmission electron microscopy(HR-TEM),and selected area electron diffraction(SAED).Based on these Au NPs,the acute myocardial infarction rapid test kit(AMIRTK)was successfully fabricated through process optimization and its performance was investigated in detail.It was found that as-synthesized Au NPs exhibited a polycrystalline structure and ultrasmall size(4.57±0.3 nm).Obtained AMIRTK with the above Au NPs could achieve the rapid detection and showed a very low limit of detection(0.1 ng/mL)for cardiac myosin binding protein-C(cMyBP-C)as myocardial marker.Meanwhile,the AMIRTK had high specificity,repeatability and stability,indicating a great potential in practice.
Au nanoparticleacute myocardial infarctiontest kitcardiac myosin binding protein-Cimmunochromatography
万方数据
维普
