Establishment of a Rat Model of Premature Ovarian Failure and a Preliminary Study on a Model of Qi and Blood Deficiency with Irregular Menstruation in Chinese Medicine
Objective To establish an effective and stable rat model of premature ovarian failure,to pro-vide an experimental carrier for the follow-up study,and to investigate its theoretical basis in Chinese medicine.Methods Female SD rats were randomly divided into four groups:a blank control group,a high-dose cisplatin group(4 mg/kg,intraperitoneal injection),a medium-dose cisplatin group(3 mg/kg,intraperitoneal injec-tion),and a low-dosecisplatin group(2.5 mg/kg,intraperitoneal injection).The same volume of saline was injected intraperitoneally into the blank control group,and the rats were fed normally for 21 days after the injec-tions for 7 consecutive days.The morphology and the number of epithelial cells,the serum estradiol and follicle stimulating hormone levels,as well as the pathological changes in the ovaries of rats were examined.Results Compared with the high-dose and low-dose cisplatin groups,after continuous intraperitoneal injection of cispla-tin for 7 days,the hair of the rats was dishevelled and lustrous,the lips and PAWS were pale,the estrus cycle was significantly changed,the serum estradiol was significantly reduced,the follicle-stimulating hormone was significantly increased,the mature follicles of the ovaries were significantly reduced,and the atretic follicles were increased,and the survival rate was 100％.Conclusion A moderate dose of cisplatin(3 mg/kg)intraper-itoneally for 7 days is a more convenient,time-saving,and stable method for creating a rat model of premature ovarian failure(POF)compared to other modeling methods.At the same time,this experimental model is highly consistent with the syndrome of deficiency of qi and blood in traditional Chinese medicine,so it is suggested that cisplatin 3 mg/kg intraperitoneally for 7 days be used as the basic modeling method for POF with syndrome of deficiency of qi and blood in traditional Chinese medicine.
Premature ovarian failuredeficiency of qi and bloodcisplatinanimal model
万方数据
维普
