Screening and Identification of Pathogen Causing Root Rot in Pseudostellaria heterophylla and Selection of Biocontrol Agents
Objective As an important Chinese medicinal herb,P.heterophylla is susceptible to root rot disease,causing significant damage to its yield and quality.This study aims to screen biocontrol agents for the root rot disease in P.heterophyllag,in order to provide a theoretical basis for future biological control methods.Methods The pathogenic fungi were isolated and purified from the diseased roots by using Koch's postulates to verify the pathogenicity.Simultaneously,rhizosphere soil from healthy P.heterophyllag was used to screen for antagonistic bacteria by using the plate confrontation method,and their species were identified.Results Fusar-ium oxysporum was isolated and identified as the pathogenic fungus causing root rot of P.heterophyllag.It was observed that Fusarium oxysporum invaded through wounds on the epidermis or root surfaces more rapidly than through stomatal invasion.Additionally,one strain exhibiting biocontrol efficacy against Fusarium oxysporum was isolated,further identified through molecular techniques as B.thuringiensis.Conclusion In this study,the pathogen of root rot of Radix pseudostellariae and its antagonistic bacteria were successfully screened,which laid a foundation for large-scale production in the later stage.
万方数据
维普
