贵州林业科技2024,Vol.52Issue(1) :11-15,41.

鲜油茶籽贮藏过程中的微观结构及酶活特性变化

Microstructure and Enzyme Activity Evolution in Fresh Camellia oleifera Seeds During Storage

刘亚娜 耿阳阳 王纪辉 胡伯凯 张时馨
贵州林业科技2024,Vol.52Issue(1) :11-15,41.

鲜油茶籽贮藏过程中的微观结构及酶活特性变化

Microstructure and Enzyme Activity Evolution in Fresh Camellia oleifera Seeds During Storage

刘亚娜 1耿阳阳 1王纪辉 1胡伯凯 1张时馨1
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作者信息

  • 1. 贵州省核桃研究所,贵州 贵阳 550005
  • 折叠

摘要

为探究鲜油茶籽在贮藏过程中的微观结构和酶活特性变化,以黔产油茶籽为试验对象,利用扫描电子显微镜跟踪鲜油茶籽在贮藏期间的微观结构,利用酶标仪测定过氧化物酶、过氧化氢酶、多酚氧化酶、脂氧合酶、脂肪酶、乙醇脱氢酶活性变化,最后进行相关性分析.结果表明:随着贮藏时间的延长,鲜油茶籽细胞组织结构出现萎缩和坍塌,细胞内含物逐渐减少;酶的活性响应变化各不相同,多酚氧化酶及脂肪酶活性持续增高,脂氧合酶活性逐渐降低;过氧化氢酶和过氧化物酶协同发挥作用,清除因活性氧代谢失衡产生的自由基进而减缓鲜油茶籽品质劣变.研究结果为油茶籽贮藏过程中的质量调控提供科学依据.

Abstract

Camellia oleifera has been widely distributed in the low hilly areas of southern China.This study aims to explore the microstructure and enzyme activity of fresh Camellia oleifera seeds during storage.Camellia oleifera seeds were taken from Guizhou Province of China.A scanning electron microscopy(SEM)was used to characterize the microstructure evolution of fresh Camellia oleifera seeds during storage.A microplate reader was used to measure the activities of peroxidase,catalase,polyphenol oxidase,lipoxygenase,lipase and alcohol dehydrogenase.Finally,the correlation analysis was carried out on the parameters.The results showed that there were the withered and collapsed microstructures of cells in the fresh Camellia oleifera seeds during storage,as the duration increased,while the con-tents of cells decreased gradually.There was the different variation in the response to enzyme activity.The activities of polyphenol oxidase and lipase increased significantly,whereas,there was the gradual decreasing in the activity of lipoxygenase.Catalase and peroxidase shared the synergistic effects.The quality deterioration of fresh Camellia oleif-era seeds was also reduced to remove the free radicals that produced by the imbalance of active oxygen metabolism.The finding can provide a scientific basis for the quality control of Camellia oleifera seeds during storage.

关键词

鲜油茶籽/贮藏/微观结构/酶活

Key words

fresh Camellia oleifera abel./storage/microstructure/enzyme activity

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基金项目

贵州省基础研究计划(黔科合基础[2020]1Y141号)

贵州省木本粮油加工科技创新人才团队(黔科合平台人才[2020]5003号)

出版年

2024
贵州林业科技
贵州省林业科学研究院 贵州省林学会

贵州林业科技

影响因子:0.37
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参考文献量14
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