Microstructure and Enzyme Activity Evolution in Fresh Camellia oleifera Seeds During Storage
Camellia oleifera has been widely distributed in the low hilly areas of southern China.This study aims to explore the microstructure and enzyme activity of fresh Camellia oleifera seeds during storage.Camellia oleifera seeds were taken from Guizhou Province of China.A scanning electron microscopy(SEM)was used to characterize the microstructure evolution of fresh Camellia oleifera seeds during storage.A microplate reader was used to measure the activities of peroxidase,catalase,polyphenol oxidase,lipoxygenase,lipase and alcohol dehydrogenase.Finally,the correlation analysis was carried out on the parameters.The results showed that there were the withered and collapsed microstructures of cells in the fresh Camellia oleifera seeds during storage,as the duration increased,while the con-tents of cells decreased gradually.There was the different variation in the response to enzyme activity.The activities of polyphenol oxidase and lipase increased significantly,whereas,there was the gradual decreasing in the activity of lipoxygenase.Catalase and peroxidase shared the synergistic effects.The quality deterioration of fresh Camellia oleif-era seeds was also reduced to remove the free radicals that produced by the imbalance of active oxygen metabolism.The finding can provide a scientific basis for the quality control of Camellia oleifera seeds during storage.