贵州林业科技2024,Vol.52Issue(2) :1-5,18.

硫氢化钠对青钱柳叶活性成分含量和体外抗氧化能力的影响

Effect of Sodium Hydrosulfide on Active Ingredients and Antioxidant Capacity of Cyclocarya paliurus Leaves

杭紫莹 陈淑慧 刘洋 杨万霞
贵州林业科技2024,Vol.52Issue(2) :1-5,18.

硫氢化钠对青钱柳叶活性成分含量和体外抗氧化能力的影响

Effect of Sodium Hydrosulfide on Active Ingredients and Antioxidant Capacity of Cyclocarya paliurus Leaves

杭紫莹 1陈淑慧 1刘洋 1杨万霞2
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作者信息

  • 1. 浙江农林大学暨阳学院,浙江 诸暨 311800
  • 2. 南京林业大学林学院,江苏 南京 210037
  • 折叠

摘要

通过探究硫化氢(H2S)供体硫氢化钠(NaHS)对青钱柳叶生物活性成分含量和体外抗氧化能力的影响,验证外源H2S是否可以提高青钱柳的药用价值.以一年生青钱柳幼苗为试验材料,在人工气候室控制条件下,设置对照组(CK)、NaHS1(0.1 mM)、NaHS2(0.5 mM)、NaHS3(1.0 mM)4 个水平处理,测定不同浓度的NaHS处理下青钱柳叶活性成分含量和抗氧化能力的变化;结果表明:(1)外源NaHS处理后青钱柳叶的总黄酮类活性成分的含量显著下降,总多糖和总三萜类活性成分的含量均无明显变化;(2)青钱柳叶总多酚类活性成分的含量及其ABTS和DPPH的自由基清除率均显著提高,同时,ABTS和DPPH的自由基清除率与总多酚类活性成分的含量呈显著正相关(P<0.05).适当浓度的NaHS处理可以增强青钱柳的药用价值.

Abstract

This study aims to explore the effect of sodium hydride(NaHS,a hydrogen sulfide,H2 S donor)on the bioactive ingredients and antioxidant capacity of leaves.Exogenous H2 S solution was then verified to improve the me-dicinal value of Cyclocarya paliurus leaves.The experimental materials were taken as one-year old C.paliurus seed-lings.Four treatments were set as NaHS1(0.1 mM),NaHS2(0.5 mM),and NaHS3(1.0 mM),and the CK(control check)with different concentrations of NaHS.Artificial climate chamber was utilized to determine the varia-tion in the active ingredient contents and antioxidant capacity of C.paliurus leaves under the control conditions.The results showed that:(1)There was the decrease in the content of total flavonoids in the leaves of Cyclocarya paliurus after exogenous NaHS treatment.While there was no significant change in the content of total polysaccharides and triterpenoids.(2)The free radical clearance rates of ABTS and DPPH were significantly positively correlated with the content of total polyphenolic active ingredients(P<0.05)in the leaves of Cyclocarya paliurus,indicating the great increase.Therefore,the optimal concentration of NaHS can be expected to enhance the medicinal value of Cy-clocarya paliurus.

关键词

青钱柳/H2S/活性成分/抗氧化能力

Key words

Cyclocarya paliurus/hydrogen sulfide/active ingredient/antioxidant capacity

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基金项目

国家自然科学基金(32001305)

浙江农林大学暨阳学院大学生创新基金(JYKC2230)

出版年

2024
贵州林业科技
贵州省林业科学研究院 贵州省林学会

贵州林业科技

影响因子:0.37
ISSN:
参考文献量38
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