多星韭AwCHI1的克隆与分析及真核表达载体的构建
Cloning and analysis of chalcone isomerase gene from Allium wallichii and its eukaryotic expression vector construction
赵鑫 1张艳 1肖松 1黄菊 1陈瑶 1孙威2
作者信息
- 1. 贵州师范大学生命科学学院/植物生理与发育调控重点实验室,贵州贵阳 550025
- 2. 贵州师范大学生命科学学院/植物生理与发育调控重点实验室,贵州贵阳 550025;西南喀斯特山地生物多样性保护重点实验室,贵州贵阳 550025
- 折叠
摘要
查尔酮异构酶(Chalcone isomerase,CHI)是类黄酮物质合成途径中的关键酶,能催化柚皮素查尔酮生成柚皮素,进而转化生成各种类型的黄酮类化合物.根据多星韭(Allium wullichii)转录组测序结果,运用PCR技术克隆获取多星韭查尔酮异构酶的编码基因(AwCHI1),对基因序列进行分析,同时与真核表达载体pBI121进行连接,将其转入农杆菌GV3101感受态细胞中,完成农杆菌的转化.研究结果不仅为该基因的功能解析研究奠定了基础,也为多星韭类黄酮代谢途径的研究提供了重要基因资源.
Abstract
Chalcone isomerase is a key enzyme in flavonoid synthesis pathway,which can catalyze the generation of naringenin from naringenin chalcone and convert it into various types of flavonoids.Aw-CHI1was successfully obtained from Allium wallichii by PCR based on the transcriptome data.Mean-while,its sequence was analyzed by using bioinformatics method and subcloned into eukaryotic expres-sion vector pBI121 which was transfered into the competent cells of Agrobacterium tumefaciens GV3101 to complete tromsformation.These results not only laid the foundation for the functional analysis of Aw-CHI1,but also provided gene resources for the study of the synthesis pathway of flavonoids in A.walli-chii.
关键词
类黄酮/查尔酮异构酶/多星韭/真核表达载体Key words
flavonoid/chalcone isomerase/Allium wallichii/eukaryotic expression vector引用本文复制引用
基金项目
贵州师范大学学术新苗基金项目([202]B04)
喀斯特山地生态安全工程研究中心(黔教合KY字[2021]007)
贵州省高等学校高山杜鹃病虫害绿色防控重点实验室项目(黔教技[2022]044号)
出版年
2024
NETL
NSTL
万方数据