Cloning and analysis of chalcone isomerase gene from Allium wallichii and its eukaryotic expression vector construction
Chalcone isomerase is a key enzyme in flavonoid synthesis pathway,which can catalyze the generation of naringenin from naringenin chalcone and convert it into various types of flavonoids.Aw-CHI1was successfully obtained from Allium wallichii by PCR based on the transcriptome data.Mean-while,its sequence was analyzed by using bioinformatics method and subcloned into eukaryotic expres-sion vector pBI121 which was transfered into the competent cells of Agrobacterium tumefaciens GV3101 to complete tromsformation.These results not only laid the foundation for the functional analysis of Aw-CHI1,but also provided gene resources for the study of the synthesis pathway of flavonoids in A.walli-chii.
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