Identification and bioinformatics analysis of ARP genes in foxtail millet(Setaria italica)
The actin-related proteins(ARPs)are homologous to the conventional actin,and nucleus ARP are regarded as components of chromatin remodeling complex.Chromatin remodeling complexes play important roles in regulation of gene transcriptional expression,and involve in the development processes in plant.In order to reveal the function of ARPs in foxtail millet,the ARP members in foxtail millet were identified by sequence alignment with the five nuclear localization genes in Arabidopsis,which are SiARP4,SiARP5,SiARP6,SiARP7 and SiARP9.Then gene structure,physicochemical prop-erties,conserved protein motifs and other traits were analyzed and predicted.The real-time quantitative PCR(RT-qPCR)was used to detect the expression level of SiARP4 and SiARP7 under different stres-ses.The results showed that the five members were hydrophilic protein and there were some difference in the composition of the conserved motifs,but all contained Actin-like ATPase domain.The phyloge-netic tree revealed that SiARP protein had a more closely relationship with ARP proteins in maize and sorghum.The promoters of ARPs in foxtail millet contain light response element,hormone response,stress response and other regulatory elements.The result of RT-qPCR showed that expression levels of ARP4 and ARP7 changed in different degrees under drought stress and salt stress,indicating that ARP4 and ARP7 involved in stress response.This study provided theoretical support for further analysis on re-sponding mechanism of chromatin remodeling component ARPs to stress resistance of millet.
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万方数据
