Gene chip analysis of the effects of lactate acid on murine immature dendritic cells
The study aims to investigate the effect of lactate acid on the genomic expression levels of immature dendritic cells(imDCs)in mice,providing preliminary data for further understanding the mo-lecular mechanisms underlying the impact of pathological acidic microenvironments on the immune function of imDCs.mononuclear cells were isolated from C57BL/6J mouse bone marrow to induce their differentiation into imDCs.By treating imDCs with 20 mmol/L concentration of lactic acid for 24 h,fol-lowed by gene chip analysis using the BGISEQ platform,differentially expressed genes were identified.The results showed that treatment with a concentration of 20 mmol/L lactic acid resulted in changes in the expression of 915 genes in imDCs.Through Gene ontology(GO)analysis,it was found that the dif-ferentially expressed genes were involved in processes such as cell cytoskeleton,apoptosis,and immune response.Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis revealed that the differentially expressed genes were related to signaling pathways including MAPK signaling pathway,PI3 K-AKT sig-naling pathway,and Toll-like receptor signaling pathway.A network diagram of interactions among dif-ferentially expressed proteins was constructed using by using Cytoscape software,and key differentially expressed proteins were filtered based on node connectivity.Up-regulated key differentially expressed proteins such as cluster of differentiation 38(CD38)and 3-hydroxy-3-methylglutaryl-coenzyme A re-ductase(HMGCR)were identified,along with down-regulated key differentially expressed proteins such as chemokine c-c-motif receptor 7(CCR7)and signal transducer and activator of transcription 1(STAT1).
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