Determination of Erythritol,Xylitol,Sorbitol,and Maltitol in Gummies by Ultrahigh Performance Liquid Chromatography-Mass Spectrometry Method
Establish an ultra-high performance liquid chromatography-mass spectrometry detection method for detecting erythritol,xylitol,sorbitol,and maltitol content in gummies.The sample was extracted by water ultrasound at 70℃for 30 min,diluted with 70%acetonitrile-water(V/V),and analyzed by ultra-high performance liquid chromatography-mass spectrometry(UPLC-QDa).The mobile phase is acetonitrile-0.1%ammonia aqueous solution,gradient elution at a flow rate of 0.2 mL/min and column temperature at 40℃,and the sample injection volume is 1 μL.Sugar alcohol was qualitatively determined by retention time and selected ions and quantified by external standard method.The linear relationship of erythritol,xylitol,sorbitol,and maltitol is good(R2＞0.99)in the concentration range of 5～160 μg/mL.The spiked recoveries are within the range of 90.44%～104.46%.The relative standard deviation(RSD)of method precision is 1.84%～4.19%(n=6).This method is applicable for the determination of erythritol,xylitol,sorbitol,and maltitol in gummies.
万方数据
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