Objective To assess the expression of miR-412 in human melanoma and investigate how miR-412 modulates melanoma cell proliferation and invasive capacity by inhibiting SRY-Box Transcription Factor 6,(SOX6)expression.Methods Analysis of the TCGA(The Cancer Genome Atlas)database identified aberrant miR-412 expression in melanoma.To explore its relevance to tumorigenesis,we conducted Transwell chamber and non-adherent independent growth assays to examine the effects of miR-412 on melanoma cell proliferation and invasion.Software predictions highlighted SOX6 as a potential target gene.We performed luciferase reporter assays and Western blot experiments to elucidate the regulatory interactions between SOX6 and miR-412.Results TCGA database analysis revealed significantly elevated miR-412 expression levels in melanoma tissues compared to the normal control group.Moreover,higher miR-412 expression correlated with shorter survival times.Functional assays using Transwell chambers and non-adherent independent growth assays demonstrated that overexpressing miR-412 enhanced cell proliferation and invasive capabilities.Conversely,reducing miR-412 expression restrained these attributes in melanoma cells.Target prediction analysis indicated that miR-412 binds to the 3'-UTR region of SOX6,resulting in decreased SOX6 protein levels due to increased miR-412 expression.Intriguingly,inhibiting SOX6 expression concurrently amplified the proliferation and invasive potential of melanoma cells,which was initially dampened by miR-412 downregulation.Conclusions Elevated miR-412 expression augments melanoma cell proliferation and invasive capabilities,while its suppression diminishes these traits.Through its targeted regulation of SOX6,miR-412 exerts a significant influence on melanoma cell proliferation and invasion.These findings underscore the pivotal role of miR-412 in melanoma pathogenesis and underscore its potential as a promising therapeutic target.
维普
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