Objective To investigate the genotypes of human papillomavirus(HPV)infection.Methods A total of 1 137 male patients'diagnoses were collected and analyzed retrospectively,which came from the detections using polymerase chain reaction reverse dot blot hybridization to genotype 28 HPV.Results Among 1 137 male patients,441 were HPV positive,with a positive rate of 38.79%,the infections of top five HPV types were HPV6(11.35%),HPV11(7.92%),HPV16(5.10%),HPV52(3.52%),HPV43(2.64%).The majority of the patients were the 20-39 age group,and the number of infections was also the highest.There was no statistical significance on the difference in the positive rate among different age groups(P>0.05).The positive rate of HPV52 in≥50 years old group was higher than the groups of aged 20~29(P<0.05)and 30~39(P<0.05).The single and multiple infections accounted for 67.35%and 32.65%.The low-risk HPV accounted for the highest proportion(41.27%)in single infections,while in patients with multiple infections,the proportion of dual infections was the largest(19.50%)and the high-and low-risk HPV mixed infections was the maximum of the infection types(15.87%).Conclusions The detection rate of positive HPV in 1 137 male patients was 38.79%,mainly were type 6,type 11,type 16,type 52 and type 43,and the single low-risk HPV infected was common.Positive rates were similar among different age groups.