Effects of Calcium Stress and Drought Stress on the Growth of Indigofera stachyodes Seedlings
In order to explore the effects of CaCl2 stress and drought stress on the growth of Indigo-fera stachyodes seedlings,different concentrations of CaCl2 solution and different drought environ-ments were used to conduct stress experiments on Indigofera stachyodes seedlings.The results showed that under drought stress conditions,the total biomass of Indigofera stachyodes seedlings in-creased with the increase of soil water content.The optimal relative field water content for the total chlorophyll content of Indigofera stachyodes was 60%-65%.Under extreme drought conditions,the content of malondialdehyde(MDA)and free proline(Pro)was the lowest,and catalase(CAT)activity was the strongest.In the environment of appropriate calcium concentration(5-35 mmol/L),the growth and development of embryonic roots,main roots,fresh weight and other growth indica-tors of Indigofera stachyodes seedlings could be promoted.When the calcium concentration was excessive,it will had an inhibitory effect instead.In the range of appropriate calcium stress concentra-tion(5-35 mmol/L),the content of MDA and chlorophyll increased,and the activity of superoxide dismutase(SOD),peroxidase(POD),free proline(Pro)and CAT increased.The comprehensive analysis showed that both too high and too low calcium ion concentrations will have an inhibitory effect on the growth of Indigofera stachyodes seedlings.Although the seedlings had certain drought resistance,as the degree of drought deepens,the growth of seedlings will be inhibited.Therefore,in the process of introducing and breeding Indigofera stachyodes,attention should be paid to appropri-ate water conservation,supplementation and control of calcium ion concentration.
Indigofera stachyodescalcium stressdrought stressgrowth indicesphysiological indices
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