To study the effects of different planting densities on the growth and yield of Rheum in high cold and damp region,and to provide support for the standardized cultivation technology of Rheum.Three different planting densities(plant spacing×row spacing)were designed:20 cm×60cm,30 cm×60 cm,40 cm×60 cm.Random block design was adopted,and 3 replicates were set for each density.The results showed that planting density had an effect on the growth and yield of Rheum.With the increase of plant distance,the measured values of each index of medicinal materials in-creased.When the plant spacing was 30 cm and the row spacing was 60 cm,the growth indexes of Rhubarb were the best,and the fresh root weight and dry root weight reached the maximum.The growth index and yield of Rheum were significantly different with different transplanting density(p<0.05),and the growth index was positively correlated with the yield.