Study on the Physiological Mechanism of Asparagus cochinchinensis Seed Germination in Response to Drought Stress
In order to analyze the effects of drought stress on the germination of Asparagus cochinchinensis seeds,the physiological characteristics of A.cochinchinensis seeds under drought stress were observed.The seeds were treated with Hoagland nutrient solution containing different PEG-6000 concentrations(0,3%,6%,9%,and 12%)to observe the physiological changes of the seeds.The results showed that under PEG-6000 treatment,the seed germination rate and water content decreased gradually compared with the control.Malondialdehyde content,electrical conductivity,hydrogen peroxide,superoxide anion and proline content,and superoxide dismutase activity increased with the increase of treatment concentration.The total soluble sugar content and the activity of other antioxidant enzymes increased with the increase of the treatment con-centration when PEG-6000 concentration was lower than 9%,but decreased when PEG-6000 concentration was higher than 9%.This indicated that drought stress could inhibit the germination of A.cochinchinensis seeds.Under low drought stress,the seed cells regulated osmotic balance by synthesizing proline and soluble sugar,and cleared excessive reactive oxygen species by antioxidant enzymes.However,most antioxidant enzyme activities were inhibited when the cells were subjected to high drought stress.
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