Based on EST-SSR Molecular Marker Designing to Identify G.e lata Bl.f.glauca S.Chow and G.e lata Bl.f.elata
This study used MISA software to screen SSR sites from the transcriptome of G.elata Bl.f.elata,then analyzed the SSR characteristics.Primers for PCR amplification and polyacrylamide gel electrophoresis test,which was used for clustering analysis,with 25 G.elata samples were designed.Results showed 12 744 EST-SSR sites were screened out,with a incidence rate of 27.9%.The main repeat types of EST-SSR were mono-,di-and tri-nucleotide,meanwhile the total number of com-pound and multi-compound repeat types was 1 071,accounting of 8.41%.Primers were designed,40 pairs random primers were used for verification by PCR and polyacrylamide gel electrophoresis,that with the result of 4 pair primers amplification showed polymorphism.These SSRs were all compound or multi-compound types with 3 allele,2.31 effective allele,Shannon'diversity index 0.69-1.33,Nei's diversity index 0.449 2-0.678 4.The amplified bands of manually read,with the analysis of PCoA,cluster and STRUCTURE,could classify the 25 samples into G.elata Bl.f.glauca S.Chow and G.elata Bl.f.elata.
万方数据
维普
