In order to obtain the homozygote of Artemisia annua with the same characters and avoid the appearance of uneven artemisia annua in the field,we conducted a study on cutting and inducing rooting of tissue culture seedlings of Artemisia annua.Three kinds of medium,MS,1/2 MS and 1/4 MS,and different NAA concentration,were used to induce the cutting roots of the stem segment with no top and stem segment with noggin.It was found that 1/2 MS medium was the best medium,the concentration of NAA was 0.3 mg/L as the optimal hormone dosage,and the culture time was 22 days,and the maximun was 30 days.