To innovate kiwifruit germplasm,the distant hybridization was cayyied out using cultivar'Mizao No.2'of A.arguta as female parent and'Moshan No.3'of A.deliciosa as male parent.The embryo rescue was performed using hy-brid immature embryos,and the effects of different embryo age and medium on embryo rescue were analyzed.The results indicated that the immature embryos after 35 days of pollination on ⑨ medium can induce multiple shoots and embryonic callus,and the induction rates of both were the highest,10％and 13.33％,respectively.The rooting effect of multiple shoots on 1/2 MS medium with 0.5 mg·L-1IBA was better.Through the embryo rescue of immature hybrid embryos,the distant hybrid offspring were successfully obtained,and the new germplasm of kiwifruit was innovated,which provided the basis for kiwifruit breeding.
维普
万方数据
