Establishment of DNA Extraction Method From Leaves of Aesculus chinensis var.wilsonii
Extracting high-quality genomic DNA is one of the essential prerequisites for conducting molecular biology research.Due to the high content of polysaccharides and polyphenols in the leaves of A.chinensis var.wilsonii,the quality of DNA ex-traction is often low,which seriously affects subsequent molecular biology operations.This study used mature leaves of 600,50,and 4-year-old A.chinensis var.wilsonii plants as materials to establish a suitable method for extracting DNA from A.chinensis var.wilsonii(M1)and compared it with three standard plant DNA extraction methods(M2-M4).The results showed that the concentration range for M1 extraction of DNA ranged from 207.19 to 488.98 ng·μL-1,the av-erage value was 308.42 ng·μL-1;the purity A260/A280 ratio ranged from 1.90 to 1.98,with an average value of 1.93.The A260/A230 ratio ranged from 1.72 to 1.95,with an average value of 1.86;DNA electrophoresis bands were clear,with mod-erate brightness,and the sample holes were clean and pollution-free;ISSR-PCR can amplify PCR products with clear bands,good stability and high polymorphism.The DNA extracted by M1 is significantly superior to the other three meth-ods(M2-M4)in terms of concentration,purity,DNA bands,and ISSR-PCR amplification products,making it suitable for the extraction of DNA from leaves of A.chinensis var.wilsonii.
万方数据
维普
