In this study,Fragaria × ananassa cv.'Benihopp'stolon tip was used as experimental material.After primary and successive culture,the virus-free seedlings were obtained.The results showed that the optimal medium formula for cal-lus induction was MS+NAA 1.0 mg·L-1+6-BA 5 mg·L-1;the optimal medium formula for callus proliferation was MS+NAA 1 mg·L-1+6-BA 10 mg·L-1+TDZ 1 mg·L-1;the optimal medium formula for inducing germination was MS+NAA 0.40 mg·L-1+6-BA 10 mg·L-1+activated carbon 1 g·L-1;the most suitable site for callus induction was leaf;the most suitable site for germination was the shoot apex;the optimal medium formula for inducing callus from leaf tissue was MS+NAA 0.50 mg·L-1+TDZ 20 mg·L-1+activated carbon 1 g·L-1;the optimal medium formula for in-ducing root growth from shoot apex was MS+IBA 5 mg·L-1+activated carbon 3 g·L-1;the optimal medium formula for rooting was 1/2MS+IBA 5 mg·L-1+activated carbon 3 g·L-1.
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