Bursaphelenchus xylophilus is a worldwide quarantine object and has caused serious harm to China's forestry.Due to the small size and simple structure of it,it is difficult to use traditional morphological identification.Therefore,ob-jective molecular biological identification methods are particularly important.In this paper.the main PCR detection tech-niques of B.xylophilus in recent years and their differences from B.mucronatus were summarized.The specific se-quences,fragment sizes and application scenarios of various detection techniques such as conventional PCR,PCR-PFLPs detection,RAPD and SCAR molecular marker and fluorescent PCR technology were described.The advantages and disad-vantages of each detection technology are discussed.It provides a reference for later research.
维普
万方数据