Effect of Tujia Medicine Ganqi Recipe on Spleen Immunity in Rats with Liver Stagnation and Spleen Deficiency
Objective To study the regulatory effect of Tujia medicine Ganqi recipe on spleen im-munity in rats with liver depression and spleen deficiency and its mechanism,so as to provide experimental basis for clinical application.Method 60 male sprague-dawley rats were randomly divided into the normal group(NG),liver stagnation and spleen deficiency model group(MG),Ganqi recipe high,middle and low dose groups(GRHG,GRMG,GRLG)and fluoxetine group(FG),with 10 rats in each group.All the five groups except the normal group were modeled by multiple factors for 21 days to replicate the model of liver stagnation and spleen deficiency.The normal group and the model group were given 0.9%NaCl solution at the same dose,and the other groups were given corresponding solution by gavage once a day for 14 days.The rats were killed on the 2nd day after the last administration,and the expression of chemokines CCL20 and CXCL8 in the spleen were detected by ELISA,the protein and gene expression of NF-κb,Bcl-2 and Bax were detected by immunohistochemistry and RT-PCR.Results The re-sults of ELISA method showed that the contents of CCL20 and CXCL8 in the high,medium and low dose groups were significantly reduced,the expression levels of NF-κB and Bax proteins and mRNA were significantly decreased,while the expression levels of Bcl-2 proteins and mR-NA were significantly increased(P<0.05 or P<0.01).Conclusion Tujia medicine Ganqi recipe can enhance splenic cell immunity and inhibit cell apoptosis by up-regulating the expression of cel-lular immunity-related factor Bcl-2 in spleen tissues,down-regulating the content of CCL20,CXCL8,and the expression of NF-κB and Bax proteins and mRNAs,and indirectly improving the effect of liver depression and spleen deficiency.
Tujia medicineQifangliver depression and spleen deficiencyimmunityapopto-sis
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