Determination of Effective Components in Rhizoma Atractylodis Chinensis and Rhizoma Atractylodis with bran-stired
Objective To establish a method for simultaneous determination of four main chemi-cal components in Rhizoma atractylodis chinensis,atractylosin,Cineole,and catechol in Rhizoma atractylodis and Rhizoma atractylodis bran-stired,in order to provide the quality evaluation basis of Rhizoma atractylodis and Rhizoma atractylodis bran-stired.Methods The mobile phase,sta-tionary phase,column temperature and flow rate were considered together.After several optimi-zations,the optimal chromatographic conditions were as follows:column temperature,25℃;de-tection wavelength,320 nm;and the control products of atractylodin,atractylodinone,eucalyptol,maitake alcohol and danpihenol were weighed on an electronic balance and made into a solution by adding methanol with the concentrations of danpihenol(12 μg/mL),atractylodinone(17 μg/mL),atractylodinone(450 μg/mL),atractylodinone(17 μg/mL)and atractylodinone(450 μg/mL),respectively,and processed by cold immersion and sonication for 30min.The peak ar-eas were compared by cold immersion and ultrasonication for 30 min.Ultrasonic extraction method was used to compare the peak areas of methanol solvent extracts at different time in-tervals.Results The results showed that the peak area of atractylosin,atractylosin,Eucalyptus al-cohol and Herba Palmatine was stable at different time points.The contents of essential oil such as atractylosin,Curcumol and eucalyptus alcohol increased obviously,while the content of atrac-tylosin decreased after processing.Conclusion The increased trend of atractylosin,curcutanol and Cineole in the volatile oil of Rhizoma atractylodis bran-stired might be related to the stron-ger spleen-strengthening effect and the weaker dryness of the Rhizoma atractylodis bran-stired.
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