Mechanism of Biosynthesized Selenium-Nanoparticles in Treating Non-Alcoholic Fatty Liver Disease
Objective To investigate the effects and mechanisms of Bio-synthesized Selenium-nanoparticles on non-alcoholic fatty liver disease(NAFLD)in mice.Methods Thirty male C57BL/6J mice were used to induce NAFLD in mice using a high-fat,low-methionine,and choline-deficient(CDAHFD)diet.The mice were randomly divided into three groups:the blank group(Con,normal feeding for 6 weeks),the model group(CDAHFD,CDAHFD diet feeding for 6 weeks and PBS gavage),and the treatment group(CDAHFD+SeNPs,CDAHFD diet feed-ing for 6 weeks and 7.5 μg/20 g nano-selenium gavage once a week for 6 weeks).The weight changes of the mice were recorded.After the end of the experimental period,the mice were fasted for 12 hours,dissected for sample collection,and the serum liver function and lipid indica-tors were detected.The liver was stained with HE,oil red O,and Masson staining for observing the pathological morphology,lipid deposition,and the degree of liver fibrosis.qRT-PCR was uti-lized to detect the levels of fat metabolism and inflammatory cytokines.Results Compared with the Con group,the liver function indicators of ALT and AST in the model group were significant-ly increased(P<0.01),as were the blood lipid indicators of TG and TB(P<0.01).The liver weight and liver coefficient were also significantly increased(P<0.01).Histological sections of the liver showed a large number of cells swollen and deformed,red lipid deposits,and collagen fibers.The mRNA expression of lipid metabolism and inflammatory factors was increased(P<0.05).Compared with the model group,the liver function indicators of ALT and AST,as well as the blood lipid indicators of TG and TB,were significantly decreased(P<0.01)in the nano-sele-nium treatment group.The liver weight and liver coefficient were also significantly lower(P<0.01).Histological sections of the liver showed a reduction in cell swelling and deformation,a decrease in lipid deposits,and a lower degree of fibrosis.The mRNA expression of lipid metabo-lism and inflammatory factors was also decreased(P<0.05).Conclusions Biosynthesized nano-selenium can effectively ameliorate NAFLD in mice induced by a high-fat diet,reduce lipid dep-osition,liver inflammation and fibrosis.
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