Study on Transformation of Tomato Mutant ‘Chloronerva’ with MxNasl Gene of Apple
To optimize the transformation system of MxNasl gene of apple into tomato mutant ‘Chloronerva’, factors such as the concentration ratioof ZT, 6-BA and IAA, concentration of Kan, the Agrobacterium concentration, infiltration time and transformation style were assembled. The results showed that the best transformation system was ZT 2.0mg/L+IAA 0.2mg/L,10-fold-dilution, 5-minute-infiltation of Agrobacterium solution, Kan 50mg/L. The successful transformation rate was up to 25％. By complementation, it was demonstrated that MxNasl was able to rescue the phenotype of the NA synthesis-defective tomato mutant ‘Chloronerva’, suggesting that it was a functional NA synthase.
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