Construction of Expression Vector for Candidate Genes of New Rice Blast Resistance Gene Pita2
Rice blast is one of the most devastating diseases of rice, and a serious threat to rice production. Exploring new rice blast resistance genes for breeding disease resistance strains is an important strategy. To study the function of two candidate genes Pita2-1 and Pita2-2, long-range PCR(LR-PCR) was employed to amplify the candidate genes from genomic DNA of the resistant cultivar PiNo.4. The appropriate products of Pita2-1(6.1 kb)and Pita2-2(16.5 kb) were purified and then ligated into the binary vector pCAMBIA1300 respectively. The positive clones were confirmed by colony PCR, restriction endonuclease digestion and sequencing, thus laid a foundation for dissecting the function of gene Pita2.
NETL
NSTL
万方数据
维普
