比色法测定刺槐花中总皂苷含量
Determination of Total Saponins in Robinia pseudoacacia L. Flowers by Colorimetry
计红芳 1张令文 2杨铭铎 3李斌 1颜振敏 1张帆1
作者信息
- 1. 河南科技学院食品学院,河南新乡453003
- 2. 河南科技学院食品学院,河南新乡453003;哈尔滨商业大学中式快餐研发中心博士后科研基地,哈尔滨150076
- 3. 哈尔滨商业大学中式快餐研发中心博士后科研基地,哈尔滨150076
- 折叠
摘要
建立了比色法测定刺槐(Robinia pseudoacacia L.)花提取物总皂苷含量的方法.以人参皂苷Rg1作为对照品,通过正交设计对香草醛-高氯酸比色法的试验条件进行优化,确定最佳比色条件为:60℃水浴挥干溶剂后,加入0.2 mL 7.0％香草醛-冰乙酸液和1 mL高氯酸,于60℃水浴20 min,测定波长为545nm.样品在0～0.24 mg范围内呈良好的线性关系,回归方程为y=3.547 0x+0.010 9(R2=0.998 9),平均加样回收率为100.07％(RSD=2.10％,n=7),测得刺槐花提取物中总皂苷平均含量为2.177％.该方法简便、准确、重现性与稳定性较好,可用于测定刺槐花提取物总皂苷含量.
Abstract
Study was carried out to establish the method of determining saponins content in Robinia pseudoacacia L. Flowers (RPF) by colorimetry. With Ginsenoside Rgl as standard material, the condition of vanillin-perchloric acid colorimetry was optimized by orthogonal design. The suitable conditions for colorimetry were obtained as follows: the sample solution was e-vaporated by water bath heating at 60℃, and was mixed with 0.2 mL acetic acid containing 7.0% vanillin and 1 mL perchloric acid, and reaction mixture was incubated at 60 ℃ for 20 min. Detection wavelength was 545 nm. A good linear relationship was achieved in the range of 0-0.24 mg with the regression equation of y=3.547 0x+0.010 9 (R2=0.998 9), and the average recovery was 100.07%(RSD=2.10% ,n=7). The average content of total saponins in RPF was 2.177%. This method was simple, accurate, excellently stable and repeatable, and was suitable for the determination of total saponins in RPF.
关键词
刺槐(Robinia/pseudoacacia/L.)花/总皂苷/含量测定/比色法Key words
Robinia pseudoacacia L. Flowers/total saponins/determination of content/colorimetry引用本文复制引用
基金项目
河南科技学院高层次人才科研项目(7041)
出版年
2012
国家科技期刊平台
NSTL
维普
万方数据