Study on the Correlation between the Leaf Flue-Curing Characteristics and Polyphenol Oxidase Activity of Different Flue-Cured Tobacco
The polyphenol oxidase (PPO) activity in tobacco leaf of different positions (lower,middle,upper) was determined during the flue-curing process from 8 flue-curing varieties.The results showed that PPO activity change of tobacco leaves from all the varieties were in the same pattern,it showed as a parabola way.The PPO activity was stronger in the later yellowing stage,and went lower gradually during color fixing stage.There was significant difference between the leaves of different positions and there was significant difference among different varieties too.There was a close relationship between the PPO activity in tobacco leaf and the leaf flue-curing characteristics of different flue-cured tobacco varieties.It showed a significant positive correlation between the PPO activity in the lower and upper leaves of different varieties and the proportion of variegated tobacco cured leaves.Different flue-curing characteristics of different flue-cured tobacco varieties could be due to differences of PPO activity in tobacco leaves.When the mean PPO activity (72 h) during the flue-curing process in the lower and middle tobacco leaves was at 0.3[△OD520/(g·min)] below,and upper leaves in 0.4[△OD520/(g·min)] below,the leaf color fixing characteristics was ideal;When the mean PPO activity (72 h) during the flue-curing process in the lower and middle tobacco leaves was at 0.4[△OD520/(g·min)]above,and upper leaves at 0.5[△OD520(g·min)] above,the leaf color fixing characteristics was not ideal.