Study on Viability and Storage Life of Heleocharis dulcis Pollen
To study the pollen viability and in vitro germination conditions of Heleocharis dulcis(Burm,f.) Trin.ex Hensch.,viability of H.dulcis pollen was determined by three testing staining methods including I2-KI,TTC,MTT and in vitro culture method.Dynamic changes of in vitro pollen germination rate under the storage condition of 25 ℃ and 4 ℃ was compared.The result indicated that the pollen dyed rate of MTT staining method was similar to the in vitro germination rate,thus the MTT staining method is suitable for fast test of the pollen viability of H.dulcis.The best medium for in vitro pollen germination of H.dulcis was 1％ sucrose + 50 μg/mL boric acid.The in vitro pollen germination rate of H.dulcis decreased to 0.29％ when stored at 25 ℃ for 50 min,while decreased to 0 when stored at 4 ℃ for 5 h.
Heleocharis dulcis (Burm.f.)Trin.ex Hensch.pollen viabilitydetermination methodstorage life
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万方数据
维普
