Cloning and Bioinformatics Analysis of RghBNG-like Gene in Rehmannia glutinosa
RghBNG gene is a novel abiotic stress and plant growth regulator-responsive gene from Rehmannia glutinosa,while RghBNGLgene is RghBNG-like gene homologous to RghBNG gene at the nucleotide level. RghBNG-like gene was successfully cloned from Rehmannia glutinosa,and predicted byabioinformatics softwares. The total RNA was extracted from Rehmannia glutinosa leaves with RNA extraction kit,and then used as reverse transcriptional template to clone RghBNG-like gene with the same primer pair as that for RghBNG gene. RghBNG-like gene was 1 974 bp long,including a 486 bp ORF,encoding for 161 amino acid residues. The protein isoelectric point and molecular weight are 9.45 and 18.6 kDa. Its secondary structure consists of 34.78℅ extended strand,33.54℅ random coil,21.74℅ alpha helix and 9.94℅ β-turn. It has two transmembrane helical segments and takes part in translation and fatty acid metabolism.
万方数据
