The experimental studies on the Hort16A of the shoot tip, the kiwifruit were carried out from the point of induc-tion, rooting and seedling. The results showed that the optimum concentration of growth regulators in the optimal medium was NAA 0.03 mg/L and 6-BA 1 mg/L, and the induction rate reached 92% in the combination of the concentration. When root-ing culture, the best concentration of IBA in 1/2MS medium was 0.5 mg/L,and the rooting rate reached 98%.