Study on Construction of prd29A-otsAB Expression Vector and Transformation of Nicotiana benthamiana
The rd29A promoter of Arabidopsis thaliana,otsA and otsB genes in Escherichia coli were cloned by PCR respec-tively. Afterwards,the target fragments were ligated into plasmid p2300-gfp,which would lead to the construction of p2300-prd29A-otsAB expression vector. By Agrobacterium-mediated method,the prd29A-otsAB fusion gene was introduced into Nico-tiana benthamiana,and then the transgenic Nicotiana benthamiana was obtained through the tissue culture and antibiotics screening for the first time. The experimental results can provide the reference for improving the stress resistance of plants through genetic engineering.
NETL
NSTL
万方数据
维普
