Studies on Factors Influencing Embryogenesis of Isolated Microspores of Xinjiang Turnips
In order to optimize microspore culture techniques of Brassica rapa L. and improve the embryo induction rate, the influence factors to microspore embryogenesis of Brassica rapa L. were studied through eight genotypes. Results showed that there was significant difference in embryogenesis ability among different genotypes. Among them, four genotypes yielded em-bryos successfully, which the rate reached 50℅. Genotype W4 was an ideal material for embryoid induction, and the fre-quency reached 73 embryos per 30 buds. NAdding phytohormone 6-BA and NAA in NLN-13 medium can improve the em-bryoid induction,4 ℃ pretreatment of bud for 24 h can significantly improve the production rate of microspore embryogenesis.
Xinjiang turnip(Brassica rapa L.)culture of pollenrate of embryo formation
NETL
NSTL
万方数据
维普
