fRANS HALS德国鸢尾组织培养及植株再生研究
Research on tissue culture and plantlet regeneration of Iris germanica 'fRANS HALS'
武海峰 1吕远达 2郑海霞 1赵明晶 1王家庆1
作者信息
- 1. 沈阳工学院,辽宁 抚顺 113122
- 2. 广东省农业科学院果树研究所,广州 510640
- 折叠
摘要
以德国鸢尾(Iris germanica. L)FRANS HALS叶片、球茎作为外植体,利用正交试验设计研究探讨不同激素及不同浓度组合对鸢尾愈伤组织的诱导、不定芽分化以及试管苗生根的影响,为鸢尾快速繁殖、种质资源的保存及利用提供最优的技术方法.结果表明,不同外植体灭菌时间也不同,叶片为3%次氯酸钠3 min,球茎为3%次氯酸钠5 min;鸢尾组培快繁的最适合外植体为球茎;鸢尾愈伤组织诱导和芽分化的最适培养基配方为MS+NAA 0.10 mg/L+6-BA 1.00 mg/L,生根培养基为MS+1\2MS+NAA 0.10 mg/L,试管苗移栽基质采用泥炭:珍珠岩=8:2,成活率达90%以上.
Abstract
Using the leaves and bulbs of Iris germanica ˊFRANS HALSˊ as explants, orthogonal experiment was used to study the effects of different hormones and different concentration combinations on the induction of callus, adventitious bud differentiation and rooting of test-tube seedlings to provide an optimized method for rapid propagation, preservation and utilization of germplasm resources of the iris. The results showed that the sterilization time required for different explants was different. The leaves were 3% sodium hypochlorite for 3 min and the bulbs were 3% sodium hypochlorite for 5 min. The most suitable explants for appendix tissue culture were bulbs. The optimum medium for callus induction and bud differentiation of Iris was MS+NAA 0.10 mg/L+6-BA 1.00 mg/L; rooting medium was MS+1\2MS+NAA 0.10 mg/L; the substrate was peat:perlite=8:2, and the survival rate was over 90%.
关键词
鸢尾(Iris/germanica./L)/组织培养/外植体/植株再生Key words
Iris germanica. L/tissue culture/explant/plantlet regeneration引用本文复制引用
出版年
2019
国家科技期刊平台
NSTL
维普
万方数据