首页|响应面法优化黄芩茎叶黄酮类成分提取工艺及其含量测定

响应面法优化黄芩茎叶黄酮类成分提取工艺及其含量测定

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采用单因素和响应面试验考察乙醇浓度、料液比、提取时间和提取温度对黄芩茎叶中黄酮成分提取量的影响;在此基础上，采用HPLC法测定了17个黄芩茎叶中黄酮成分的含量。结果表明，黄芩茎叶中黄酮成分的最佳提取工艺为乙醇浓度55%、料液比1∶50、提取时间60 min、提取温度50℃，应用最佳提取工艺黄酮成分提取量为24。43 mg/g;不同产地黄芩茎叶中黄酮成分的含量存在差异，含量范围为15。839～26。044 mg/g。响应面法优化黄芩茎叶中黄酮成分的提取工艺稳定、准确可靠，所建立的黄酮成分含量测定方法稳定可靠，可为黄芩茎叶的质量评价及深度开发研究提供参考。
Optimization of extraction process of flavonoids from Scutellaria baicalensis stem and leaf by response surface methodology and its content determination
The effects of ethanol concentration,solid-liquid ratio,extraction time and extraction temperature on the extraction of fla-vonoids from Scutellaria baicalensis stem and leaf were investigated by single factor and response surface experiment.On this basis,the contents of flavonoids in 17 Scutellaria baicalensis stems and leaves were determined by HPLC.The results showed that the opti-mum extraction condition of flavonoids in Scutellaria baicalensis stem and leaves was ethanol concentration of 55%,solid-liquid ratio of 1∶50,extraction time of 60 min and extraction temperature of 50℃.Under the optimal extraction process,the extraction amount of flavonoids was 24.43 mg/g.The contents of total flavonoids in Scutellaria baicalensis stem and leaf from different regions were different,ranging from 15.839 mg/g to 26.044 mg/g.The response surface methodology was stable,accurate and reliable to optimize the extrac-tion process of total flavonoids from Scutellaria baicalensis stem and leaf.The established method for the determination of flavonoids content was stable and reliable,which could provide a reference for the quality evaluation and further development of Scutellaria ba-icalensis stem and leaf.

Scutellaria baicalensis stem and leafflavonoidsextraction processhigh-performance liquid chromatography(HPLC)content determinationresponse surface methodology

闫杰、张思访、毛贤贤、许晨新、刘贝

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江苏卫生健康职业学院药学院,南京 211800

黄芩茎叶 黄酮类成分 提取工艺 高效液相色谱法(HPLC) 含量测定 响应面法

江苏卫生健康职业学院校级课题江苏省大学生创新创业训练计划项目

JKC202001202114255005Y

2024

10.14088/j.cnki.issn0439-8114.2024.01.024

湖北农业科学
湖北省农业科学院　华中农业大学　长江大学　黄冈师范学院

湖北农业科学

CSTPCD
影响因子：0.442
ISSN：0439-8114
年,卷(期)：2024.63(1)
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