首页|不同栽培条件下白花前胡质量特征分析

不同栽培条件下白花前胡质量特征分析

Quality characteristics analysis of Peucedanum praeruptorum Dunn.under different cultivation conditions

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为测定不同产地、不同种植条件下的白花前胡(Peucedanum praeruptorum Dunn.)药材中白花前胡甲素、白花前胡乙素的含量,分析影响白花前胡药材品质的原因.使用高效液相色谱法,采用C18色谱柱(4.6 mm×250 mm,5 μm),流动相为甲醇-水(体积比为75∶25),流速为1.0 mL/min,柱温为30℃,检测波长为321 nm.白花前胡甲素、白花前胡乙素含量分别为5.0～400.0 μg/L和2.5～200.0 μg/L时线性关系良好;白花前胡甲素和白花前胡乙素峰面积的相对标准偏差分别为0.04%和0.05%.研究表明,在海拔100～800 m时,白花前胡中白花前胡甲素含量随海拔变化没有明显变化,但均达《中华人民共和国药典(2020年版)》要求;白花前胡乙素含量随着海拔升高整体呈上升趋势,海拔升高,昼夜温差变大,有利于白花前胡乙素富集;相较于施用氮磷钾复合肥,动物粪便发酵有机肥能提升2种香豆素类化合物含量;在氮磷钾复合肥基础上施用微量元素肥有利于白花前胡乙素富集.
To determine the content of Peucedanum praeruptorum Dunn.A and Peucedanum praeruptorum Dunn.B in the medicinal herb Peucedanum praeruptorum Dunn.from different regions and under different planting conditions,and to analyze the factors affect-ing the quality of the medicinal herb,the high-performance liquid chromatography and a C18 column(4.6 mm×250 mm,5 μm)were used.The mobile phase was methanol-water(volume ratio of 75∶25),with a flow rate of 1.0 mL/min,column temperature of 30℃,and detection wavelength of 321 nm.When the content of Peucedanum praeruptorum Dunn.A and Peucedanum praeruptorum Dunn.B was 5.0～400.0 μg/L and 2.5～20.0 μg/L,respectively,the linear relationship was good;the relative standard deviations of peak areas for Peucedanum praeruptorum Dunn.A and Peucedanum praeruptorum Dunn.B were 0.04%and 0.05%,respectively.Research had shown that at an altitude of 100～800 meters,the content of Peucedanum praeruptorum Dunn.A in Peucedanum praeruptorum Dunn.did not show significant changes with altitude,but all met the requirements of"Pharmacopoeia of the People's Republic of China(2020 edition)";the content of Peucedanum praeruptorum Dunn.B showed an overall upward trend with increasing altitude.As the al-titude increased,the temperature difference between day and night increased,which was conducive to the enrichment of Peucedanum praeruptorum Dunn.B;compared to the application of nitrogen,phosphorus,and potassium compound fertilizers,animal manure fer-mentation organic fertilizer could increase the content of two coumarin compounds;the application of trace element fertilizer on the ba-sis of nitrogen,phosphorus and potassium compound fertilizer was beneficial for the enrichment of Peucedanum praeruptorum Dunn.B.

Peucedanum praeruptorum Dunn.high-performance liquid chromatographyPeucedanum praeruptorum Dunn.APeuce-danum praeruptorum Dunn.Bcoumarin

石丁夫、周海波、张露、于腾、陈菊移、程扶旗

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安徽省科技成果转化促进中心(安徽省科学技术研究院),合肥 230088

绩溪县农业农村水利局,安徽 绩溪 245300

白花前胡(Peucedanum praeruptorum Dunn.) 高效液相色谱法 白花前胡甲素 白花前胡乙素 香豆素

安徽省重点研究与开发计划项目安徽省重点研究与开发计划项目2021年度安徽省科技厅直属单位科技服务能力建设专项2021年池州市贵池区科技项目

202204c06020010202004b11020017202106b03020004GCKJ202107

2024

10.14088/j.cnki.issn0439-8114.2024.01.029

湖北农业科学
湖北省农业科学院　华中农业大学　长江大学　黄冈师范学院

湖北农业科学

CSTPCD
影响因子：0.442
ISSN：0439-8114
年,卷(期)：2024.63(1)
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