首页|不同栽培条件下白花前胡质量特征分析

不同栽培条件下白花前胡质量特征分析

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为测定不同产地、不同种植条件下的白花前胡(Peucedanum praeruptorum Dunn。)药材中白花前胡甲素、白花前胡乙素的含量，分析影响白花前胡药材品质的原因。使用高效液相色谱法，采用C18色谱柱(4。6 mm×250 mm，5 μm)，流动相为甲醇-水(体积比为75∶25)，流速为1。0 mL/min，柱温为30℃，检测波长为321 nm。白花前胡甲素、白花前胡乙素含量分别为5。0～400。0 μg/L和2。5～200。0 μg/L时线性关系良好;白花前胡甲素和白花前胡乙素峰面积的相对标准偏差分别为0。04%和0。05%。研究表明，在海拔100～800 m时，白花前胡中白花前胡甲素含量随海拔变化没有明显变化，但均达《中华人民共和国药典(2020年版)》要求;白花前胡乙素含量随着海拔升高整体呈上升趋势，海拔升高，昼夜温差变大，有利于白花前胡乙素富集;相较于施用氮磷钾复合肥，动物粪便发酵有机肥能提升2种香豆素类化合物含量;在氮磷钾复合肥基础上施用微量元素肥有利于白花前胡乙素富集。
Quality characteristics analysis of Peucedanum praeruptorum Dunn.under different cultivation conditions
To determine the content of Peucedanum praeruptorum Dunn.A and Peucedanum praeruptorum Dunn.B in the medicinal herb Peucedanum praeruptorum Dunn.from different regions and under different planting conditions,and to analyze the factors affect-ing the quality of the medicinal herb,the high-performance liquid chromatography and a C18 column(4.6 mm×250 mm,5 μm)were used.The mobile phase was methanol-water(volume ratio of 75∶25),with a flow rate of 1.0 mL/min,column temperature of 30℃,and detection wavelength of 321 nm.When the content of Peucedanum praeruptorum Dunn.A and Peucedanum praeruptorum Dunn.B was 5.0～400.0 μg/L and 2.5～20.0 μg/L,respectively,the linear relationship was good;the relative standard deviations of peak areas for Peucedanum praeruptorum Dunn.A and Peucedanum praeruptorum Dunn.B were 0.04%and 0.05%,respectively.Research had shown that at an altitude of 100～800 meters,the content of Peucedanum praeruptorum Dunn.A in Peucedanum praeruptorum Dunn.did not show significant changes with altitude,but all met the requirements of"Pharmacopoeia of the People's Republic of China(2020 edition)";the content of Peucedanum praeruptorum Dunn.B showed an overall upward trend with increasing altitude.As the al-titude increased,the temperature difference between day and night increased,which was conducive to the enrichment of Peucedanum praeruptorum Dunn.B;compared to the application of nitrogen,phosphorus,and potassium compound fertilizers,animal manure fer-mentation organic fertilizer could increase the content of two coumarin compounds;the application of trace element fertilizer on the ba-sis of nitrogen,phosphorus and potassium compound fertilizer was beneficial for the enrichment of Peucedanum praeruptorum Dunn.B.

Peucedanum praeruptorum Dunn.high-performance liquid chromatographyPeucedanum praeruptorum Dunn.APeuce-danum praeruptorum Dunn.Bcoumarin

石丁夫、周海波、张露、于腾、陈菊移、程扶旗

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安徽省科技成果转化促进中心(安徽省科学技术研究院),合肥 230088

绩溪县农业农村水利局,安徽 绩溪 245300

白花前胡(Peucedanum praeruptorum Dunn.) 高效液相色谱法 白花前胡甲素 白花前胡乙素 香豆素

安徽省重点研究与开发计划项目安徽省重点研究与开发计划项目2021年度安徽省科技厅直属单位科技服务能力建设专项2021年池州市贵池区科技项目

202204c06020010202004b11020017202106b03020004GCKJ202107

2024

10.14088/j.cnki.issn0439-8114.2024.01.029

湖北农业科学
湖北省农业科学院　华中农业大学　长江大学　黄冈师范学院

湖北农业科学

CSTPCD
影响因子：0.442
ISSN：0439-8114
年,卷(期)：2024.63(1)
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