To determine the content of Peucedanum praeruptorum Dunn.A and Peucedanum praeruptorum Dunn.B in the medicinal herb Peucedanum praeruptorum Dunn.from different regions and under different planting conditions,and to analyze the factors affect-ing the quality of the medicinal herb,the high-performance liquid chromatography and a C18 column(4.6 mm×250 mm,5 μm)were used.The mobile phase was methanol-water(volume ratio of 75∶25),with a flow rate of 1.0 mL/min,column temperature of 30℃,and detection wavelength of 321 nm.When the content of Peucedanum praeruptorum Dunn.A and Peucedanum praeruptorum Dunn.B was 5.0～400.0 μg/L and 2.5～20.0 μg/L,respectively,the linear relationship was good;the relative standard deviations of peak areas for Peucedanum praeruptorum Dunn.A and Peucedanum praeruptorum Dunn.B were 0.04%and 0.05%,respectively.Research had shown that at an altitude of 100～800 meters,the content of Peucedanum praeruptorum Dunn.A in Peucedanum praeruptorum Dunn.did not show significant changes with altitude,but all met the requirements of"Pharmacopoeia of the People's Republic of China(2020 edition)";the content of Peucedanum praeruptorum Dunn.B showed an overall upward trend with increasing altitude.As the al-titude increased,the temperature difference between day and night increased,which was conducive to the enrichment of Peucedanum praeruptorum Dunn.B;compared to the application of nitrogen,phosphorus,and potassium compound fertilizers,animal manure fer-mentation organic fertilizer could increase the content of two coumarin compounds;the application of trace element fertilizer on the ba-sis of nitrogen,phosphorus and potassium compound fertilizer was beneficial for the enrichment of Peucedanum praeruptorum Dunn.B.
维普
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