Optimization of the ISSR system and primer screening for caulis spatholobi
The stem of caulis spatholobi was used as the experimental material for DNA extraction using the improved CTAB method.The ISSR-PCR reaction system was optimized and constructed using orthogonal and single factor experiments.The results showed that the optimal ISSR-PCR reaction system for caulis spatholobi was DNA template 60 ng,Taq enzyme 2.00 U,Mg2+ concentration 1.75 mmol/L,dNTP concentration 0.25 mmol/L,primer concentration 0.20 μmol/L,and optimal annealing temperature 44.6℃.20 ISSR primers suitable for caulis spatholobi from were selected 100 ISSR primers.
万方数据
维普
