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中国主要油茶产区山茶炭疽菌群体遗传结构分析

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以中国主要油茶产区的油茶(Camellia oleifera Abel。)炭疽病优势种山茶炭疽菌(Colletotrichum camelliae)的16个地理种群168个菌株为供试材料,应用ISSR分子标记技术进行遗传多样性和种群遗传结构分析。16个地理种群的多态位点百分比(PPB)为 98。99%,Nei's基因多样性指数(He)为 0。28,Shannon信息多样性指数(Is)为0。43,遗传相似度(I)平均为0。834,遗传距离(D)平均为0。183,表明山茶炭疽菌遗传多样性水平较高且异质性较强,种群间存在一定程度的遗传变异,遗传距离与地理距离之间无相关性。湖北省、浙江省、江西省、湖南省和广西5个省级种群总基因多样度(Ht)为0。274 8,遗传分化系数(Gst)为0。517 4,基因流(Nm)为0。466 4,表明山茶炭疽菌5个省级种群虽然被分化,但是不存在基因流动现象(Nm<1)。
Population genetic structure analysis of camellia anthracis fungus in the main oil tea producing areas of China
Using 168 strains from 16 geographical populations of camellia anthracis fungus(Colletotrichum camelliae Abel.),a domi-nant species of camellia anthracis in the main camellia producing areas of China,as the test materials,ISSR molecular marker technol-ogy was used to analyze genetic diversity and population genetic structure.The percentage of polymorphic loci(PPB)for 16 geographi-cal populations was 98.99%,the Nei's gene diversity index(He)was 0.28,the Shannon information diversity index(Is)was 0.43,the average genetic similarity(I)was 0.834,and the average genetic distance(D)was 0.183.This indicated that the genetic diversity level and heterogeneity of camellia anthracis fungus were high,and there was a certain degree of genetic variation among populations.There was no correlation between genetic distance and geographical distance.The total genetic diversity(Ht),genetic differentiation coefficient(Gst),and gene flow(Nm)of the five provincial-level populations in Hubei,Zhejiang,Jiangxi,Hunan,and Guangxi provinces were 0.274 8,0.517 4,and 0.466 4,respectively.This indicated that although the five provincial-level populations of ca-mellia anthracis fungus were differentiated,there was no gene flow phenomenon(Nm<1).

camellia(Camellia oleifera Abel.)anthracis fungus(Colletotrichum camelliae)ISSRgenetic diversitypopulation ge-netic structureoil tea producing areas

王义勋、徐小文、郑露、黄俊斌、谢先斌

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湖北省林业科学研究院,武汉 430075

华中农业大学植物科技学院,武汉 430070

郧西县林业局,湖北 十堰 442600

山茶(Camellia oleifera Abel.) 炭疽菌(Colletotrichum camelliae) ISSR 遗传多样性 种群遗传结构 油茶产区

湖北省重点研发计划资助项目

2020BBA042

2024

湖北农业科学
湖北省农业科学院 华中农业大学 长江大学 黄冈师范学院

湖北农业科学

CSTPCD
影响因子:0.442
ISSN:0439-8114
年,卷(期):2024.63(1)
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