摘要
利用筛选得到的复合菌系来发酵玉米秸秆,研究复合菌系对降解秸秆产短链脂肪酸的影响,并对微生物菌群结构组成进行解析.结果表明,复合菌系在前4d的降解速度最快,秸秆、纤维素和木质素的降解率分别达30.35％、27.07％和14.71％,在12d时,其降解率分别达40.02％、32.25％和20.93％.复合菌系的代谢产物以异丁酸和乙酸为主,其含量分别占总短链脂肪酸的36.46％和33.15％.由高通量测序可知,复合菌系中的优势菌属主要由 Clostridium sensu stricto、Cellulosilyticum、Clostridium XIVa、Enterobacteriaceae和Peptostreptococcaceae等组成.
Abstract
The compound strains obtained by screening were used to ferment corn straw,the effects of the compound strains on degrad-ing straw to produce short-chain fatty acids were studied,and the structural composition of the microbial consortium was analyzed.The results showed that the degradation rate of the compound strain was the fastest in the first 4 days.The degradation rates of straw,cellulose and lignin reached 30.35％,27.07％and 14.71％,respectively.At 12 days,the degradation rates reached 40.02％,32.25％and 20.93％,respectively.The metabolites of the compound strain were isobutyric acid and acetic acid,which accounted for 36.46％and 33.15％of the total short chain fatty acids,respectively.According to high-throughput sequencing,the dominant bacteria genera in the compound strains were mainly composed of Clostridium sensu stricto,Cellulosilyticum,Clostridium XIVa,Enterobacteriaceae and Peptostreptococcaceae.
基金项目
安徽省省级重点基金项目(JZ2022QSKJ0010)
维普
万方数据