Screening,identification and culture conditions optimization of a highly efficient potassium-solubilizing bacteria
Five strains of potassium-solubilizing bacteria were isolated and screened from black soil paddy field.One strain(S1)with high efficiency of potassium-solubilizing was screened by its morphological characteristics and potassium-solubilizing ability.The spe-cies of S1 was identified by 16S rDNA,and the culture conditions of the strain were optimized by the single factor test and orthogonal test.The results showed that the five strains of potassium-solubilizing bacteria were all able to decompose potassium feldspar effective-ly.Strain S1 had the highest potassium-solubilizing ability with a potassium-solubilizing rate of 57.03%,and the content of potassi-um-solubilizing in the fermentation broth was 3.852 mg/L,which was the best potassium-solubilizing bacteria.The potassium-solubi-lizing strain S1 was identified as Burkholderia ambifaria.The optimized culture conditions were carbon source 1.0%mannitol,nitrogen source 1.0%peptone,inorganic salt 0.5%K2HPO4,culture temperature 30℃,culture time 48 h,liquid volume 80 mL/250 mL,ini-tial pH 6.5,and inoculation volume 5.0%.
lack soilpotassium-solubilizing bacteriastrain identificationculture conditionsorthogonal test
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